OsNAC300 Positively Regulates Cadmium Stress Responses and Tolerance in Rice Roots

Transcriptional regulation is involved in responding to cadmium (Cd) stress plants. However, the molecular mechanisms of Cd responses regulated by transcription factors remain largely unknown In this study, a rice (Oryza sativa) NAC (no apical meristem [NAM]; Arabidopsis activation factor [ATAF]; cup-shaped cotyledon [CUC]-related) family factor, OsNAC300, was isolated and functionally characterized for its involvement tolerance. OsNAC300 localized nucleus. mainly expressed roots significantly induced treatment. Knockout resulted increased sensitivity stress, while overexpression lines enhanced tolerance stress. RNA-Seq analysis revealed that mutant impaired regulating some important genes were responsive wild-type rice, such as pathogenesis-related 10a (OsPR10a), OsPR10b, chalcone synthase 1 (OsCHS1), several others, which validated reverse quantitative polymerase chain reaction (RT-qPCR) analysis. Moreover, chromatin immunoprecipitation-qPCR assays luciferase reporter demonstrated directly binds promoters OsPR10a, OsCHS1 activates their transcription. Overall, an regulatory rice.